1DUN

EIAV DUTPASE NATIVE

Experimental procedure

Spacegroup name	H 3 2
Unit cell lengths	86.270, 86.270, 95.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.1598 *
Rwork	0.161
R-free	0.21550 *
RMSD bond length	0.022
RMSD bond angle	0.042
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *
High resolution limit [Å]	1.900 *	1.900 *
Rmerge	0.133 *	0.422 *
Total number of observations	55093 *
Number of reflections	10717 *
Completeness [%]	97.5 *	96.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7	4 *	DROP: 1.4 MG/ML PROTEIN, 0.05 M IMIDAZOL MALATE BUFFER PH 7.0, 15% PEG 3400. WELL: 0.01 M IMIDAZOL MALATE BUFFER PH 7.0, 30% PEG 3400; GROWTH TIME: ABOUT 5 MONTHS.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.5-1.6 (mg/ml)
2	1	reservoir	PEG3400	15 (%)
3	1	reservoir	imidazole/malate	0.1 (M)