1DUL
STRUCTURE OF THE RIBONUCLEOPROTEIN CORE OF THE E. COLI SIGNAL RECOGNITION PARTICLE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-30 |
| Detector | OTHER |
| Wavelength(s) | 0.9793,0.9787,0.9667 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.567, 78.291, 32.849 |
| Unit cell angles | 90.00, 96.14, 90.00 |
Refinement procedure
| Resolution | 28.830 - 1.800 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.22100 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 12.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.830 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 56313 |
| Completeness [%] | 89.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.6 * | VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | isopropanol | 10 (%) | |
| 2 | 1 | reservoir | Na-MES | 50 (mM) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | 125 (mM) | ||
| 5 | 1 | reservoir | C-HEGA 10(Anatrace) | 35 (mM) |
