1DUG
STRUCTURE OF THE FIBRINOGEN G CHAIN INTEGRIN BINDING AND FACTOR XIIIA CROSSLINKING SITES OBTAINED THROUGH CARRIER PROTEIN DRIVEN CRYSTALLIZATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03-15 |
| Detector | FUJI |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 105.780, 105.780, 137.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 - 1.800 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.22600 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.425 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.000 |
| High resolution limit [Å] | 1.700 | 1.800 |
| Rmerge | 0.054 | 0.250 |
| Total number of observations | 376772 * | |
| Number of reflections | 67736 | |
| Completeness [%] | 89.2 | 66.1 |
| Redundancy | 5.56 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | -170 * | PEG 3350, sodium acetate, sodium chloride, ammonium sulfate, Tris, reduced glutathione, pH 4.6, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 10 | 1 | reservoir | ammonium sulfate | 35 (mM) | |
| 2 | 1 | drop | PEG3350 | 8 (%) | |
| 3 | 1 | drop | sodium acetate | 50 (mM) | |
| 4 | 1 | drop | sodium chloride | 25 (mM) | |
| 5 | 1 | drop | ammonium sulfate | 17.5 (mM) | |
| 6 | 1 | drop | Tris-HCl | 5 (mM) | |
| 7 | 1 | drop | glutathinoe | 5 (mM) | |
| 8 | 1 | reservoir | PEG3350 | 16 (%) | |
| 9 | 1 | reservoir | sodium acetate | 100 (mM) |
