1DTW
HUMAN BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 143.760, 143.760, 69.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.27900 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.140 |
| Total number of observations | 306699 * |
| Number of reflections | 22910 * |
| Completeness [%] | 99.9 |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Ethanol, PEG 8000,MES, potassium chloride, magnesium chloride, glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 20K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ethanol | 35-40 (%) | |
| 10 | 1 | drop | inhibitor | 1 (mM) | |
| 2 | 1 | reservoir | PEG8000 | 0-2 (%) | |
| 3 | 1 | reservoir | MES | 0.1 (M) | |
| 4 | 1 | drop | protein | 18 (mg/ml) | |
| 5 | 1 | drop | MES | 0.1 (M) | |
| 6 | 1 | drop | 0.25 (M) | ||
| 7 | 1 | drop | glycerol | 10 (%) | |
| 8 | 1 | drop | ThDP | 1 (mM) | |
| 9 | 1 | drop | 10 (mM) |
