1DTH

METALLOPROTEASE

Experimental procedure

Spacegroup name	P 65
Unit cell lengths	97.210, 97.210, 87.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.168
Rwork	0.168
RMSD bond length	0.010
RMSD bond angle	2.190
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.063 *
Total number of observations	222723 *
Number of reflections	36261
Completeness [%]	92.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8 *		Zhang, D., (1994) Proc.Nat.Acad.Sci.USA, 91, 8447. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Ht-d	10 (mg/ml)
2	1	drop	imidazole	0.1 (M)
3	1	reservoir		20 (mM)
4	1	reservoir	ammonium sulfate	2.4 (M)