1DSU
HUMAN FACTOR D, COMPLEMENT ACTIVATING ENZYME
Experimental procedure
| Detector technology | AREA DETECTOR |
| Detector | XENTRONICS |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.990, 65.020, 40.360 |
| Unit cell angles | 101.26, 109.89, 74.32 |
Refinement procedure
| Resolution | 7.500 - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.20300 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.031 * |
| Data reduction software | XENGEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Rmerge | 0.060 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.4 * | 22 * | Narayana, S.V., (1991) J.Mol.Biol., 219, 1. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 50 (mM) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | PEG6000 | 40 (%(w/v)) | |
| 4 | 1 | drop | protein | 8.5 (mg/ml) | |
| 5 | 1 | drop | MES | 50 (mM) | |
| 6 | 1 | drop | 100 (mM) | ||
| 7 | 1 | drop | reservoir | 0.002ml. without NaCl |
