1DSO

CYTOCHROME C PEROXIDASE H175G MUTANT, IMIDAZOLE COMPLEX AT PH 6, ROOM TEMPERATURE.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	281
Detector technology	IMAGE PLATE
Collection date	1998-11-20
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	107.710, 76.830, 51.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.100 - 2.500 *
R-factor	0.17 *
RMSD bond length	0.006 *
RMSD bond angle	1.800 *
Data reduction software	XFIT
Data scaling software	CCP4 ((SCALA))
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.080	2.080
High resolution limit [Å]	2.500 *	2.030
Rmerge	0.120 *	0.726
Number of reflections	26702
<I/σ(I)>	4.3
Completeness [%]	97.0 *	66.2
Redundancy	3.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	6	288	MES, imidazole, pH 6, MICRODIALYSIS, temperature 288K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.4 (mM)
2	1	drop	phosphate	75 (mM)
3	1	drop	imidazole	10 (mM)
4	1	reservoir	MES	50 (mM)
5	1	reservoir	imidazole	10 (mM)