1DSG

CYTOCHROME C PEROXIDASE H175G MUTANT, IMIDAZOLE COMPLEX AT PH 5, ROOM TEMPERATURE.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	281
Detector technology	IMAGE PLATE
Collection date	1998-11-20
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	107.900, 76.610, 51.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	11.000 - 3.200 *
R-factor	0.16 *
RMSD bond length	0.005 *
RMSD bond angle	1.400 *
Data reduction software	XFIT
Data scaling software	CCP4 ((SCALA))
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	11.400	2.620
High resolution limit [Å]	3.200 *	2.560
Rmerge	0.160 *	0.637
Number of reflections	13358
<I/σ(I)>	1.7
Completeness [%]	96.3 *	31.7
Redundancy	4.5	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	6 *	288	MES, imidazole, pH 5.2, MICRODIALYSIS, temperature 288K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.4 (mM)
2	1	drop	phosphate	75 (mM)
3	1	drop	imidazole	10 (mM)
4	1	reservoir	MES	50 (mM)
5	1	reservoir	imidazole	10 (mM)