1DSE
CYTOCHROME C PEROXIDASE H175G MUTANT, IMIDAZOLE COMPLEX, WITH PHOSPHATE BOUND, PH 6, 100K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05-29 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.258, 73.768, 51.041 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.000 - 2.000 |
| R-factor | 0.196 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.900 |
| Data reduction software | XFIT |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.053 | 0.189 |
| Number of reflections | 38609 | |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 98.3 | 91.1 |
| Redundancy | 2.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6 | 4 * | phosphate, imidazole, pH 6, MICRODIALYSIS, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 0.4 (mM) | |
| 2 | 1 | 1 | potassium phosphate | 500 (mM) | |
| 3 | 1 | 1 | imidazole | 10 (mM) | |
| 4 | 1 | 2 | potassium phosphate | 50 (mM) | |
| 5 | 1 | 2 | imidazole | 10 (mM) |
