1DSB
CRYSTAL STRUCTURE OF THE DSBA PROTEIN REQUIRED FOR DISULPHIDE BOND FORMATION IN VIVO
Experimental procedure
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.500, 65.000, 76.500 |
| Unit cell angles | 90.00, 126.30, 90.00 |
Refinement procedure
| Resolution | 6.000 * - 2.000 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.610 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * | 21 * | Martin, J.L., (1993) J.Mol.Biol., 230, 1097. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DsbA | 11 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 20-25 (%(w/v)) | |
| 3 | 1 | reservoir | MPD | 1 (%) | |
| 4 | 1 | reservoir | cacodylate | 0.1 (M) | |
| 5 | 1 | drop | MPD | 0.5 (%) | |
| 6 | 1 | drop | PEG8000 | 10-12.5 (%(w/v)) | |
| 7 | 1 | drop | cacodylate | 0.05 (M) |
