1DS5
DIMERIC CRYSTAL STRUCTURE OF THE ALPHA SUBUNIT IN COMPLEX WITH TWO BETA PEPTIDES MIMICKING THE ARCHITECTURE OF THE TETRAMERIC PROTEIN KINASE CK2 HOLOENZYME.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.681, 119.846, 145.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 3.160 |
| R-factor | 0.214 * |
| Rwork | 0.214 |
| R-free | 0.28900 |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.600 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.350 |
| High resolution limit [Å] | 3.160 | 3.160 |
| Rmerge | 0.160 | 0.430 |
| Total number of observations | 87314 * | |
| Number of reflections | 25909 | |
| <I/σ(I)> | 3.8 | |
| Completeness [%] | 97.2 | 97.1 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 * | 292 | Guerra, B., (1998) Acta Crystallogr., Sect.D, 54, 143. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (mg/ml) | |
| 2 | 1 | drop | 167 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 8.3 (mM) | |
| 4 | 1 | drop | 2-mercaptoethanol | 2 (mM) | |
| 5 | 1 | drop | ATP | 2 (mM) | |
| 6 | 1 | drop | 0.5 (mM) | ||
| 7 | 1 | reservoir | PEG4000 | 25 (%) | |
| 8 | 1 | reservoir | sodium acetate | 200 (mM) | |
| 9 | 1 | reservoir | Tris-HCl | 100 (mM) |
