1DRZ
U1A SPLICEOSOMAL PROTEIN/HEPATITIS DELTA VIRUS GENOMIC RIBOZYME COMPLEX
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-15 |
| Detector | RIGAKU |
| Wavelength(s) | 0.9761, 0.9794, 0.9792, 1.1390 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 109.350, 109.350, 190.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.281 |
| Rwork | 0.281 |
| R-free | 0.28400 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.300 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.065 | 0.318 |
| Total number of observations | 55889 * | |
| Number of reflections | 17817 | |
| <I/σ(I)> | 21.7 | 5.2 |
| Completeness [%] | 95.7 | 97.6 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 25 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.5 (mM) | |
| 2 | 1 | drop | 1.25 (mM) | ||
| 3 | 1 | reservoir | mPEG2000 | 12.5-14 (%(w/v)) | |
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 5 | 1 | reservoir | 200-250 (mM) | ||
| 6 | 1 | reservoir | spermine-HCl | 4 (mM) |
