1DRK

PROBING PROTEIN-PROTEIN INTERACTIONS: THE RIBOSE-BINDING PROTEIN IN BACTERIAL TRANSPORT AND CHEMOTAXIS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	74.570, 88.840, 39.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 2.000
R-factor	0.174
Rwork	0.174
RMSD bond length	0.010
RMSD bond angle	23.500 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	7.500 *
High resolution limit [Å]	2.000 *
Rmerge	0.061 *
Total number of observations	72070 *
Number of reflections	17203 *
Completeness [%]	95.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG4000	32 (%(w/v))
2	1	drop	sodium phosphate	90 (mM)
3	1	drop	ribose	8 (mM)
4	1	reservoir	PEG4000	26 (%)