1DR1

2.2 ANGSTROMS CRYSTAL STRUCTURE OF CHICKEN LIVER DIHYDROFOLATE REDUCTASE COMPLEXED WITH NADP+ AND BIOPTERIN

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	89.440, 48.310, 64.260
Unit cell angles	90.00, 125.10, 90.00

Refinement procedure

Resolution	? - 2.200
R-factor	0.14
RMSD bond length	0.021
RMSD bond angle	0.040 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.022 *
Number of reflections	10688 *
Completeness [%]	91.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	cDHFR	15 (mg/ml)
2	1	drop	NADP+	2 (mM)
3	1	drop	calcium acetate	10 (mM)
4	1	drop	dithiothreitol	2 (mM)
5	1	drop	Tris-HCl	20 (mM)
6	1	reservoir	ethanol	23 (%(v/v))
7	1	reservoir	calcium acetate	10 (mM)
8	1	reservoir	Tris-HCl	20 (mM)