1DQW

CRYSTAL STRUCTURE OF OROTIDINE 5'-PHOSPHATE DECARBOXYLASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS
Synchrotron site	APS
Temperature [K]	110
Detector technology	CCD
Collection date	1999-04-18
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	90.060, 116.217, 116.979
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.218
Rwork	0.218
R-free	0.25500
RMSD bond length	0.010
RMSD bond angle	2.400
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.049	0.113
Number of reflections	70148
<I/σ(I)>	22.5
Completeness [%]	97.1	93.4
Redundancy	15.1	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	4 *	sodium-HEPES, isopropanol, polyethylene glycol 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 278.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium HEPES	0.1 (M)	pH7.0
2	1	reservoir	isopropanol	10 (%)
3	1	reservoir	PEG4000	20 (%(w/v))