1DQV

CRYSTAL STRUCTURE OF SYNAPTOTAGMIN III C2A/C2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	293
Detector technology	CCD
Collection date	1998-03-13
Detector	ADSC QUANTUM 4
Spacegroup name	P 62 2 2
Unit cell lengths	125.959, 125.959, 118.438
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 3.200
R-factor	0.293
Rwork	0.293
R-free	0.34900 *
RMSD bond length	0.008
RMSD bond angle	1.620 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.050	0.785
Total number of observations	61080 *
Number of reflections	9578
<I/σ(I)>	2.9
Completeness [%]	99.6	99.9
Redundancy	6.3	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	20 *	1.6M Magnesium Sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir		1.5 (M)
3	1	reservoir	MES	100 (mM)