1DQM

CRYSTAL STRUCTURE OF ANTI-LYSOZYME ANTIBODY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-05-05
Detector	MACSCIENCE
Spacegroup name	C 1 2 1
Unit cell lengths	162.360, 39.740, 70.290
Unit cell angles	90.00, 98.10, 90.00

Refinement procedure

Resolution	100.000 - 2.100
R-factor	0.225
Rwork	0.225
R-free	0.26900 *
RMSD bond length	0.006
RMSD bond angle	1.370 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.090	0.328
Total number of observations	88367 *
Number of reflections	26702 *
<I/σ(I)>	12.8
Completeness [%]	94.0	67.3
Redundancy	3.3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	16 (%(w/v))
3	1	reservoir		0.1 (M)
4	1	reservoir	Tris-HCl	0.1 (M)