1DQJ

CRYSTAL STRUCTURE OF THE ANTI-LYSOZYME ANTIBODY HYHEL-63 COMPLEXED WITH HEN EGG WHITE LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-05-05
Detector	MACSCIENCE
Spacegroup name	P 42 21 2
Unit cell lengths	90.580, 90.580, 150.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.000
R-factor	0.217
Rwork	0.217
R-free	0.24900 *
RMSD bond length	0.005
RMSD bond angle	26.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.089	0.371
Total number of observations	366760 *
Number of reflections	45762
<I/σ(I)>	19.6
Completeness [%]	92.7	85
Redundancy	8	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	PEG 4K, Ammonium acetate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	15 (%(w/v))
3	1	reservoir	ammonium acetate	0.1 (M)
4	1	reservoir	sodium acetate	0.05 (M)