1DQG

CRYSTAL STRUCTURE OF THE CYSTEINE RICH DOMAIN OF MANNOSE RECEPTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1998-10-01
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	39.580, 41.280, 98.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.700
R-factor	0.198
Rwork	0.196
R-free	0.20800
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	1.890
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.770
High resolution limit [Å]	1.700	1.700
Rmerge	0.055	0.205
Total number of observations	152280 *
Number of reflections	18536
<I/σ(I)>	21
Completeness [%]	98.1	91.3
Redundancy	8.2	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	4 *	PEG8000, ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	4 *	PEG8000, ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	drop		10 (mM)
4	1	reservoir	PEG8000	30 (%(w/v))
5	1	reservoir	ammonium sulfate	0.2 (M)