1DQE
BOMBYX MORI PHEROMONE BINDING PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-05-06 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 53.180, 53.180, 191.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.218 * |
| Rwork | 0.210 |
| R-free | 0.27300 |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 2.400 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.400 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.061 | 0.264 |
| Total number of observations | 135291 * | |
| Number of reflections | 26466 | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 5.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 298 | PEG20000, Tris, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ethanol | 0.65 (M) | |
| 2 | 1 | drop | protein | 20 (mg/ml) | |
| 3 | 1 | reservoir | PEG20000 | 50 (%(w/v)) | |
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) |
