1DQE

BOMBYX MORI PHEROMONE BINDING PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1999-05-06
Detector	ADSC QUANTUM 4
Spacegroup name	P 41 21 2
Unit cell lengths	53.180, 53.180, 191.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.218 *
Rwork	0.210
R-free	0.27300
RMSD bond length	0.012 *
RMSD bond angle	2.400 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.400	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.061	0.264
Total number of observations	135291 *
Number of reflections	26466
<I/σ(I)>	9.2
Completeness [%]	99.6	99.9
Redundancy	5.1	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	298	PEG20000, Tris, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ethanol	0.65 (M)
2	1	drop	protein	20 (mg/ml)
3	1	reservoir	PEG20000	50 (%(w/v))
4	1	reservoir	Tris-HCl	100 (mM)