1DQ6
Manganese;Manganese concanavalin A at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 291 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1996-07-28 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 63.200, 87.410, 89.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.900 |
| Data reduction software | ROTAVATA |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.088 | 0.241 |
| Number of reflections | 19671 | |
| <I/σ(I)> | 16.53 | 7.69 |
| Completeness [%] | 87.1 | 77.7 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 2 M ammonium sulphate, 60 mM sodium acetate at pH 7, 3% PEGMe 5000, 1 mM MnCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
