1DQ0
Locked, metal-free concanavalin A, a minor species in solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-24 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 63.006, 87.290, 88.807 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.000 - 1.700 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.20400 |
| Starting model (for MR) | 1con |
| RMSD bond length | 0.013 |
| RMSD bond angle | 27.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.061 | 0.294 |
| Total number of observations | 311946 * | |
| Number of reflections | 27215 * | |
| <I/σ(I)> | 22.4 | 5.7 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 11.5 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 293 | 2.1 M AMMONIUM SULPHATE, 120 MM SODIUM ACETATE, SOAKING OF LOCKED MNMN CON A CRYSTALS WITH THE METAL CHELATOR EDTA, pH 7.0, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | metal-free ConA | 17.3 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 2 (M) | |
| 3 | 1 | drop | 60 (mM) | ||
| 4 | 1 | drop | mPEG5000 | 3 (%) | |
| 5 | 1 | reservoir | 8 (mM) | ||
| 6 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 7 | 1 | reservoir | 60 (mM) | ||
| 8 | 1 | reservoir | mPEG5000 | 3 (%) |
