1DPW

STRUCTURE OF HEN EGG-WHITE LYSOZYME IN COMPLEX WITH MPD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	77.510, 77.510, 37.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.640
R-factor	0.1783 *
Rwork	0.178
R-free	0.22200
RMSD bond length	0.011 *
RMSD bond angle	2.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	1.700
High resolution limit [Å]	1.640	1.640
Rmerge	0.080	0.190
Total number of observations	183746 *
Number of reflections	14454
<I/σ(I)>	25.6
Completeness [%]	99.7	100
Redundancy	12.71

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5 *	293	2-METHYL-2,4-PENTANEDIOL, TRISHYDROXYMETHYLAMINOMETHANE, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	sodium acetate	50 (mM)
3	1	reservoir		5 (%(w/v))