1DPG
GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM LEUCONOSTOC MESENTEROIDES
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.88, 1.00 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 105.710, 105.710, 224.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.000 - 2.000 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25700 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.320 * |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 92804 |
| Completeness [%] | 94.0 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5-6.0 (mg/ml) | |
| 2 | 1 | drop | phosphate | 1.8-2.4 (M) | NaH2PO4 : K2HPO4 = 7.14 |
| 3 | 1 | reservoir | phosphate | 2.8-3.2 (M) |
