1DPE

DIPEPTIDE-BINDING PROTEIN

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Detector technology	IMAGE PLATE
Detector	FUJI
Spacegroup name	P 21 21 21
Unit cell lengths	118.270, 79.780, 62.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.169 *
Rwork	0.169
R-free	0.22100 *
RMSD bond length	0.014
RMSD bond angle	0.038
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.067 *
Number of reflections	38709 *
Completeness [%]	95.3 *
Redundancy	3.86

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	DppA	15 (mg/ml)
2	1	drop	sodium cacodylate	10 (mM)
3	1	reservoir	ammonium sulfate	52-55 (%)
4	1	reservoir		0.2 (M)
5	1	reservoir	Bis-Tris-propane-HCl	50 (mM)