1DPB

CRYSTALLOGRAPHIC AND ENZYMATIC INVESTIGATIONS ON THE ROLE OF SER558, HIS610 AND ASN614 IN THE CATALYTIC MECHANISM OF AZOTOBACTER VINELANDII DIHYDROLIPOAMIDE ACETYLTRANSFERASE (E2P)

Experimental procedure

Spacegroup name	F 4 3 2
Unit cell lengths	225.016, 225.016, 225.016
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.187
Rwork	0.187
RMSD bond length	0.013
RMSD bond angle	2.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.071 *
Total number of observations	52974 *
Number of reflections	13426 *
Completeness [%]	78.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	50 (mM)
3	1	drop	azide	0.02 (%)
4	1	drop	ammonium sulfate	12-16 (%)
5	1	reservoir	ammonium sulfate	12-16 (%)
6	1	reservoir	HEPES	50 (mM)