1DOZ

CRYSTAL STRUCTURE OF FERROCHELATASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	AREA DETECTOR
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	48.510, 49.970, 119.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.910 - 1.800
R-factor	0.177 *
Rwork	0.183
R-free	0.20500 *
RMSD bond length	0.005 *
RMSD bond angle	1.220 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.950	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.068	0.220
Number of reflections	27034
Completeness [%]	97.8	98.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5 *	298	used to microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	subtilis ferrochelatase	1 (mM)
2	1	drop	N-MeMP	1 (mM)
3	1	reservoir	PEG2000	30 (%(w/v))
4	1	reservoir		0.2 (M)
5	1	reservoir	Tris-HCl	100 (mM)
6	1	reservoir		0.5 (mM)