1DOL

MONOCYTE CHEMOATTRACTANT PROTEIN 1, I-FORM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-09-11
Detector	MARRESEARCH
Spacegroup name	I 41 2 2
Unit cell lengths	84.860, 84.860, 52.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.2
Rwork	0.200
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dom
RMSD bond length	0.015
RMSD bond angle	28.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.100 *	0.267 *
Number of reflections	3573
<I/σ(I)>	8.1	2.62
Completeness [%]	87.9	82.2
Redundancy	2.41	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8		10 MG/ML PROTEIN IN 50 MM TRIS BUFFER PH 7.5-8 EQUILIBRATED AGAINST 50-55% AMMONIUM SULFATE USING HANGING DROP VAPOR DIFFUSION METHOD., pH 8.0, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	reservoir	ammonium sulfate	50-55 (%(w/v))