1DO5

HUMAN COPPER CHAPERONE FOR SUPEROXIDE DISMUTASE DOMAIN II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	113
Detector technology	CCD
Collection date	1999-08-08
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	117.186, 66.716, 88.033
Unit cell angles	90.00, 96.63, 90.00

Refinement procedure

Resolution	19.210 - 2.750
R-factor	0.228
Rwork	0.228
R-free	0.28300
RMSD bond length	0.007
RMSD bond angle	25.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.830
High resolution limit [Å]	2.750	2.750
Rmerge	0.080	0.312
Number of reflections	17097
<I/σ(I)>	6.7
Completeness [%]	96.5	96.5
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	25 *	drop consists of equal amounts of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	reservoir	MES	100 (mM)
3	1	reservoir	PEG20000	12 (%)