1DO3
CARBONMONOXY-MYOGLOBIN (MUTANT L29W) AFTER PHOTOLYSIS AT T>180K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 105 |
Detector technology | AREA DETECTOR |
Collection date | 1998-11-08 |
Detector | SIEMENS HI-STAR |
Spacegroup name | P 6 |
Unit cell lengths | 90.460, 90.460, 45.260 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.000 - 1.550 |
R-factor | 0.193 * |
Rwork | 0.193 |
R-free | 0.21800 |
Starting model (for MR) | 1do1 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | SAINT ((SIEMENS)) |
Data scaling software | SAINT ((SIEMENS)) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 1.600 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.046 | 0.166 |
Total number of observations | 190950 * | |
Number of reflections | 29266 | |
<I/σ(I)> | 14 | 4.2 |
Completeness [%] | 95.1 | 84.1 |
Redundancy | 6.5 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.5 * | 292 | CRYSTALS WERE GROWN IN 2.5M AMMONIUM SULFATE SOLUTION, BUFFERED WITH 20MM TRIS/ HCL TO PH 8.5. THE CRYSTALLIZATION SOLUTION WAS REPLACED IN STEPS AGAINST A SOLUTION CONTAINING 2.5M AMMONIUM SULFATE, 20MM TRIS, 300MG/ML TREHALOSE AT PH 8.5., BATCH CRYSTALLIZATION, temperature 292K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | L29W MbCO | 20 (mM) | |
2 | 1 | 1 | glycerol | 75 (%) | |
3 | 1 | 1 | potassium phosphate | 25 (%(v/v)) | |
4 | 1 | 2 | ammonium sulfate | 2.5 (M) | |
5 | 1 | 2 | Tris-HCl | ||
6 | 1 | 2 | trehalose | 300 (mg/ml) |