1DNZ
A-DNA DECAMER ACCGGCCGGT WITH MAGNESIUM BINDING SITES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-05-05 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 38.502, 38.502, 77.631 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.229 * |
| Rwork | 0.227 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 0.025 |
| Data scaling software | d*TREK |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.042 | 0.489 |
| Number of reflections | 4901 | |
| <I/σ(I)> | 47.8 | 9.2 |
| Completeness [%] | 99.6 | 96.8 |
| Redundancy | 15.2 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 298 | MPD, CACODYLATE, MGCL2, SPERMINE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | CACODYLATE | ||
| 2 | 1 | 1 | SPERMINE | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | DNA duplex | 5 (mM) | 0.0025ml | |
| 2 | 1 | sodium cacodylate | 100 (mM) | 0.0010ml | |
| 3 | 1 | MPD | 20 (%) | 0.005ml | |
| 4 | 1 | spermine | 15 (mM) | 0.0025ml | |
| 5 | 1 | 1 | 100 (mM) | 0.0025ml |
