1DNC
HUMAN GLUTATHIONE REDUCTASE MODIFIED BY DIGLUTATHIONE-DINITROSO-IRON
Experimental procedure
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1996-08 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.080, 63.400, 84.700 |
Unit cell angles | 90.00, 58.33, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.700 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.24800 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.520 |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 1.740 | |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.093 * | 0.329 * |
Total number of observations | 239537 * | |
Number of reflections | 58100 | |
<I/σ(I)> | 14.7 | 2 |
Completeness [%] | 97.0 | 75 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 120 (mM) | |
3 | 1 | drop | potassium phosphate | 100 (mM) | |
4 | 1 | drop | beta-octyl glucoside | 0.1 (%) | |
5 | 1 | reservoir | ammonium sulfate | 720 (mM) | |
6 | 1 | reservoir | potassium phosphate | 100 (mM) |