1DMB

REFINED 1.8 ANGSTROMS STRUCTURE REVEALS THE MECHANISM OF BINDING OF A CYCLIC SUGAR, BETA-CYCLODEXTRIN, TO THE MALTODEXTRIN BINDING PROTEIN

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	38.860, 44.320, 58.310
Unit cell angles	101.50, 99.30, 102.20

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.21
Rwork	0.210
RMSD bond length	0.014
RMSD bond angle	0.031 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.730 *
Rmerge	0.030 *
Total number of observations	63892 *
Number of reflections	32945 *
Completeness [%]	87.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2 *	4 *	used to seed *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG6000	20-25 (%)
2	1	drop	MBP	6.7 (mg/ml)
3	1	drop	MES	20 (mM)
4	1	drop	beta-cyclodextrin	0.17 (mM)
5	1	reservoir	PEG6000	25-30 (%)