1DM9
HEAT SHOCK PROTEIN 15 KD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 160 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09-01 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 113.070, 67.930, 41.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.28560 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.608 |
| Data reduction software | bioteX |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | 0.353 |
| Number of reflections | 21673 | 1911 * |
| <I/σ(I)> | 7.23 | |
| Completeness [%] | 98.4 | 88.9 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 298 | drop consists of equal volume of protein and precipitant solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | HEPES | 50 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.0 (M) | precipitant |
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) | precipitant |
| 7 | 1 | reservoir | glycerol | 10 (%) | precipitant |
