1DM2

HUMAN CYCLIN-DEPENDENT KINASE 2 COMPLEXED WITH THE INHIBITOR HYMENIALDISINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1997-02-05
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	53.390, 70.670, 72.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.192
Rwork	0.192
R-free	0.26700
RMSD bond length	0.008
RMSD bond angle	25.360 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.048	0.223
Number of reflections	16264
<I/σ(I)>	21.2
Completeness [%]	98.1	86.6
Redundancy	3.2	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4	4 *	Rosenblatt, J., (1993) J. Mol. Biol., 230, 1317. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	drop	EDTA	1 (mM)
4	1	reservoir	HEPES	200 (mM)
5	1	reservoir	EDTA	1 (mM)