1DLW

X-RAY CRYSTAL STRUCTURE OF TRUNCATED HEMOGLOBIN FROM P.CAUDATUM.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-02-19
Detector	MARRESEARCH
Spacegroup name	P 43
Unit cell lengths	61.180, 61.180, 35.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.540
R-factor	0.133
Rwork	0.133
R-free	0.18300
RMSD bond length	0.014
RMSD bond angle	1.570
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.570
High resolution limit [Å]	1.540	1.540
Rmerge	0.025	0.106
Number of reflections	36762
<I/σ(I)>	31
Completeness [%]	96.7	88.2 *
Redundancy	3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.5 *	4 *	AMMONIUM SULPHATE, SODIUM ACETATE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	ammonium sulfate	35 (%)
3	1	reservoir	sodium acetate	50 (mM)