1DLU
UNLIGANDED BIOSYNTHETIC THIOLASE FROM ZOOGLOEA RAMIGERA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-07-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.712, 79.726, 150.477 |
| Unit cell angles | 90.00, 93.07, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.212 * |
| Rwork | 0.212 |
| R-free | 0.26300 |
| RMSD bond length | 0.020 * |
| RMSD bond angle | 3.200 * |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.210 |
| Rmerge | 0.090 | 0.204 |
| Number of reflections | 93668 | |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 98.3 | 89.5 |
| Redundancy | 4.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 294 | AMMONIUM SULPHATE, LITHIUM SULPHATE, SODIUM ACETATE, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 0.9 (M) | |
| 2 | 1 | reservoir | lithium sulfate | 1 (M) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 4 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | sodium azide | 1 (mM) | |
| 6 | 1 | reservoir | EDTA | 1 (mM) |
