1DLF
HIGH RESOLUTION CRYSTAL STRUCTURE OF THE FV FRAGMENT FROM AN ANTI-DANSYL SWITCH VARIANT ANTIBODY IGG2A(S) CRYSTALLIZED AT PH 5.25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 112 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-08 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.101, 70.722, 53.464 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.450 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.23000 |
| Structure solution method | MULTIPLE MOLECULAR REPLACEMENT |
| Starting model (for MR) | FV PORTION OF FAB' B13I2 (1IGF) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 28.290 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.067 | 0.265 |
| Total number of observations | 280945 * | |
| Number of reflections | 36158 | |
| <I/σ(I)> | 35.3 | 8.3 |
| Completeness [%] | 93.9 | 94.4 |
| Redundancy | 8.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.25 | 293 * | pH 5.25 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 0.8 (M) | |
| 3 | 1 | drop | sodium cacodylate | 25 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 5 | 1 | reservoir | sodium cacodylate | 25 (mM) |
