1DLE

FACTOR B SERINE PROTEASE DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1997-04-22
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	51.643, 74.259, 73.258
Unit cell angles	90.00, 95.55, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.21
Rwork	0.209
R-free	0.24900
RMSD bond length	0.009
RMSD bond angle	1.430
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.069	0.315
Number of reflections	32252
<I/σ(I)>	19
Completeness [%]	99.2	94.1
Redundancy	3.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	298	drop consists of equal amounts of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		100 (mM)
4	1	reservoir	PEG1000	35 (%)
5	1	reservoir	MES	30 (mM)