1DKX

THE SUBSTRATE BINDING DOMAIN OF DNAK IN COMPLEX WITH A SUBSTRATE PEPTIDE, DETERMINED FROM TYPE 1 SELENOMETHIONYL CRYSTALS

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	94.500, 116.600, 37.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 2.000
R-factor	0.206
Rwork	0.206
R-free	0.28800
RMSD bond length	0.008
RMSD bond angle	24.000 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000	2.000 *
Rmerge	0.052 *	0.185 *
Total number of observations	59575 *
Number of reflections	13579
Completeness [%]	94.9	84.6 *
Redundancy	37

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		or dialysis *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.4 (M)
2	1	reservoir	phosphate