1DK5

CRYSTAL STRUCTURE OF ANNEXIN 24(CA32) FROM CAPSICUM ANNUUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	1998-11-09
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	96.760, 96.760, 173.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.216
Rwork	0.216
R-free	0.31600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	19.300 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.960
High resolution limit [Å]	2.800	2.800
Rmerge	0.077	0.484
Number of reflections	23400
<I/σ(I)>	7.5	1.5
Completeness [%]	98.3	91.8
Redundancy	4.5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5	288	1.7 M (NH4)2SO4, 2 mM CaCl2, 0.1 M NaAc , pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 15K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	1.7 (M)
2	1	1	sodium acetate	0.1 (M)
3	1	1		2 (mM)