1DK4
CRYSTAL STRUCTURE OF MJ0109 GENE PRODUCT INOSITOL MONOPHOSPHATASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1998-07-14 |
Detector | UCSD MARK II |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.870, 78.430, 129.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.600 |
R-factor | 0.186 * |
Rwork | 0.242 |
R-free | 0.33100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1awb |
RMSD bond length | 0.014 |
RMSD bond angle | 0.021 |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.080 | 0.410 * |
Number of reflections | 21539 | |
<I/σ(I)> | 9.3 | 1.1 |
Completeness [%] | 98.3 * | 95.5 * |
Redundancy | 3.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | PEG 8000, calcium chloride, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 8 (mg/ml) | |
2 | 1 | drop | PEG8000 | 5.5 (%(w/v)sat) | |
3 | 1 | drop | Tris | 25 (mM) | |
4 | 1 | drop | 50 (mM) | ||
5 | 1 | drop | 5 (mM) | ||
6 | 1 | drop | phosphate | 5 (mM) | |
7 | 1 | reservoir | PEG8000 | 11-15 (%(w/v)) |