1DK1
DETAILED VIEW OF A KEY ELEMENT OF THE RIBOSOME ASSEMBLY: CRYSTAL STRUCTURE OF THE S15-RRNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 128.800, 128.800, 65.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.29100 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.038 | 0.209 |
| Number of reflections | 8164 | |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 8.1 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.2 | 20 * | drop:reservoir in a 3:2 volume ratio * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 2 | 1 | 1 | KCL | ||
| 3 | 1 | 1 | (NH4)2SO4 | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 2 | KCL | ||
| 6 | 1 | 2 | (NH4)2SO4 | ||
| 7 | 1 | 2 | MGCL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 6 (mg/ml) | |
| 2 | 1 | drop | S15 | 3 (mg/ml) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
| 4 | 1 | reservoir | 1.5 (mM) | ||
| 5 | 1 | reservoir | 50 (mM) | ||
| 6 | 1 | reservoir | sodium cacodylate | 50 (mM) |
