1DJZ
PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C-DELTA1 FROM RAT COMPLEXED WITH INOSITOL-4,5-BISPHOSPHATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-03-30 |
| Detector | MARRESEARCH |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 396.490, 396.490, 396.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.950 |
| R-factor | 0.212 * |
| Rwork | 0.212 |
| R-free | 0.27000 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.640 |
| Data reduction software | MOSFLM |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 * | |
| High resolution limit [Å] | 2.950 * | |
| Rmerge | 0.091 | 0.326 * |
| Total number of observations | 326969 * | |
| Number of reflections | 55796 | |
| Completeness [%] | 94.8 | 92.8 * |
| Redundancy | 5.9 | 4.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 12 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | CHAPSO | 0.1 (%(w/v)) | |
| 3 | 1 | drop | ammonium phosphate | 2.0 (M) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | ammonium phosphate | 1.45 (M) | |
| 6 | 1 | reservoir | dithiothreitol | 1 (mM) |
