1DJW

PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C-DELTA1 FROM RAT COMPLEXED WITH INOSITOL-2-METHYLENE-1,2-CYCLIC-MONOPHOSPHONATE

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Detector technology	IMAGE PLATE
Collection date	1995-11-04
Detector	MARRESEARCH
Spacegroup name	F 41 3 2
Unit cell lengths	397.030, 397.030, 397.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.450
R-factor	0.215 *
Rwork	0.217
R-free	0.27000
RMSD bond length	0.012 *
RMSD bond angle	1.287 *
Data reduction software	MOSFLM
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.000 *
High resolution limit [Å]	2.450 *
Rmerge	0.067	0.316 *
Total number of observations	495077 *
Number of reflections	95932
Completeness [%]	98.5	97.4 *
Redundancy	5.2	4.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		12 *	macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	CHAPSO	0.1 (%(w/v))
3	1	drop	ammonium phosphate	2.0 (M)
4	1	drop	dithiothreitol	1 (mM)
5	1	reservoir	ammonium phosphate	1.45 (M)
6	1	reservoir	dithiothreitol	1 (mM)