1DJN

STRUCTURAL AND BIOCHEMICAL CHARACTERIZATION OF RECOMBINANT WILD TYPE TRIMETHYLAMINE DEHYDROGENASE FROM METHYLOPHILUS METHYLOTROPHUS (SP. W3A1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1995-12-03
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 1 21 1
Unit cell lengths	148.160, 72.000, 83.810
Unit cell angles	90.00, 97.73, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.139
Rwork	0.139
R-free	0.18500
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	SDMS
Data scaling software	SDMS
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.320
High resolution limit [Å]	2.220	2.220
Rmerge	0.052	0.132
Number of reflections	81423
<I/σ(I)>	17
Completeness [%]	93.8	70.9
Redundancy	3.2	2.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	295	PEG 8000, PHOSPHATE BUFFER, SODIUM CHLORIDE, pH 6.5, VAPOR DIFFUSION, HANGING DROP AND MICROSEEDING, temperature 295K