1DJI
PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C-DELTA1 FROM RAT COMPLEXED WITH CALCIUM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11-16 |
| Detector | MARRESEARCH |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 398.030, 398.030, 398.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.22 * |
| Rwork | 0.220 |
| R-free | 0.28000 |
| Structure solution method | DIFFERENCE FOURIER |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Phasing software | CCP4 |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 * | |
| High resolution limit [Å] | 2.500 * | |
| Rmerge | 0.073 | 0.296 * |
| Total number of observations | 397693 * | |
| Number of reflections | 90470 | |
| Completeness [%] | 98.0 | 97.1 * |
| Redundancy | 4 | 4.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 12 * | Essen, L.O., (1996) Nature, 380, 595. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium phosphate | 1.4 (M) | |
| 2 | 1 | drop | protein | 5 (mg/ml) | |
| 3 | 1 | drop | Tris-HCl | 5 (mM) | |
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | EGTA | 0.25 (mM) | |
| 6 | 1 | drop | DTT | 0.5 (mM) | |
| 7 | 1 | drop | sodium azide | 0.005 (%(w/v)) | |
| 8 | 1 | drop | CHAPSO | 0.05 (%(w/v)) | |
| 9 | 1 | drop | ammonium phosphate | 0.7 (M) |
