1DJ8
CRYSTAL STRUCTURE OF E. COLI PERIPLASMIC PROTEIN HDEA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-12-05 |
| Detector | OTHER |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.000, 73.600, 74.300 |
| Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.26100 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.730 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.123 |
| Number of reflections | 32222 | |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 97.2 | 97.7 |
| Redundancy | 8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4 | 298 | PEG1500, SODIUM ACETATE, pH 4, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 5 (mm) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 4 | 1 | reservoir | PEG1500 | 26-30 (%(w/v)) |
