1DIH

THREE-DIMENSIONAL STRUCTURE OF E. COLI DIHYDRODIPICOLINATE REDUCTASE

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	75.700, 81.200, 94.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.186 *
Rwork	0.187
RMSD bond length	0.020
RMSD bond angle	2.800
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.077 *
Total number of observations	76793 *
Number of reflections	15200 *	2346 *
Completeness [%]	93.4 *	74.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.2 (M)
2	1	reservoir	HEPES	100 (mM)
3	1	drop	protein	18 (mg/ml)
4	1	drop	ammonium sulfate	2.2 (M)
5	1	drop	HEPES	100 (mM)