1DI2

CRYSTAL STRUCTURE OF A DSRNA-BINDING DOMAIN COMPLEXED WITH DSRNA: MOLECULAR BASIS OF DOUBLE-STRANDED RNA-PROTEIN INTERACTIONS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	1996-01-25
Detector	RIGAKU RAXIS IIC
Spacegroup name	C 1 2 1
Unit cell lengths	110.400, 58.500, 58.900
Unit cell angles	90.00, 105.20, 90.00

Refinement procedure

Resolution	15.000 - 1.900
R-factor	0.231
Rwork	0.228
R-free	0.25800
Structure solution method	SIR
RMSD bond length	0.006
RMSD bond angle	15.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	1.960
High resolution limit [Å]	1.890	1.890
Rmerge	0.063	0.287
Number of reflections	26083
<I/σ(I)>	10	2.5
Completeness [%]	90.8	50
Redundancy	2.4	1.66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	298	PEG 4000, ETHYLENE GLYCOL, KCL, NACL, MES, DTT, BETA-MERCAPTOETHANOL, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	KCL
2	1	1	NACL
3	1	1	MES
4	1	1	DTT
5	1	1	BETA-MERCAPTOETHANOL
6	1	1	ETHYLENE GLYCOL
7	1	1	PEG 4000
8	1	2	ETHYLENE GLYCOL
9	1	2	PEG 4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Xlrbpa-2	0.7 (mM)
2	1	drop	r(GGCGCGCGCC)	0.7 (mM)
3	1	reservoir		225 (mM)
4	1	reservoir		50 (mM)
5	1	reservoir	PEG4000	1 (%)
6	1	reservoir	ehthylene glycol	12 (%)
7	1	reservoir	MES	100 (mM)
8	1	reservoir	dithiothreitol	10 (mM)
9	1	reservoir	beta-mercaptoethanol	10 (mM)